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NDUFA12 Rabbit pAb

Anti-NDUFA12 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218151

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218151
Product Name:   Anti-NDUFA12 Rabbit Polyclonal Antibody
Synonyms:   B17.2; DAP13
UNIPROT ID:   Q9UI09 (Gene Accession – BC005936 )
Background:    This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 218151(NDUFA12 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the fusion protein and then with 218151(Anti-NDUFA12 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 218151(Anti-NDUFA12 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D223830(Anti-NDUFA12 Antibody) at dilution 1/30.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human fetal muscle tissue, PC-3 and Jurkat cell;
Primary antibody: 218151(NDUFA12 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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