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SFN Rabbit pAb

Anti-SFN Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218211

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218211
Product Name:   Anti-SFN Rabbit Polyclonal Antibody
Synonyms:   YWHAS
UNIPROT ID:   P31947 (Gene Accession – BC000329 )
Background:    This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.
Immunogen:    Fusion protein of human SFN
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 200-400;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 218211(SFN Antibody) at a dilution of 1/170(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 218211(Anti-SFN Antibody) at dilution 1/170.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 218211(Anti-SFN Antibody) at a dilution of 1/170.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D223958(Anti-SFN Antibody) at dilution 1/170.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: LOVO, HT-29, Hela cell, Mouse lung tissue, 293T, HepG2 cell lysates;
Primary antibody: 218211(SFN Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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