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MIF4GD Rabbit pAb

Anti-MIF4GD Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218217

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218217
Product Name:   Anti-MIF4GD Rabbit Polyclonal Antibody
Synonyms:   MIFD; AD023; SLIP1
UNIPROT ID:   A9UHW6 (Gene Accession – BC033759 )
Background:    This gene encodes a protein which interacts with the N-terminus of the stem-loop binding protein (SLBP) and the 3′ end of histone mRNA. This interaction facilitates the activation of histone mRNA translation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen:    Fusion protein of human MIF4GD
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 218217(MIF4GD Antibody) at a dilution of 1/70(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 218217(Anti-MIF4GD Antibody) at dilution 1/70.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cell lysate;
Primary antibody: 218217(MIF4GD Antibody) at dilution 1/550;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 seconds
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