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ZYX Rabbit pAb

Anti-ZYX Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218262

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218262
Product Name:   Anti-ZYX Rabbit Polyclonal Antibody
Synonyms:   ESP-2; HED-2
UNIPROT ID:   Q15942 (Gene Accession – BC008743 )
Background:    Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.
Immunogen:    Fusion protein of human ZYX
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 218262(ZYX Antibody) at a dilution of 1/30(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 218262(Anti-ZYX Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cell lysate;
Primary antibody: 218262(ZYX Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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