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WDFY1 Rabbit pAb

Anti-WDFY1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218282

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218282
Product Name:   Anti-WDFY1 Rabbit Polyclonal Antibody
Synonyms:   WDF1; FENS1; FENS-1; ZFYVE17
UNIPROT ID:   Q8IWB7 (Gene Accession – BC040525 )
Background:    The protein encoded by this gene is a phosphatidylinositol 3-phosphate binding protein, which contains a FYVE zinc finger domain and multiple WD-40 repeat domains. When exogenously expressed, it localizes to early endosomes. Mutagenesis analysis demonstrates that this endosomal localization is mediated by the FYVE domain.
Immunogen:    Fusion protein of human WDFY1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 218282(WDFY1 Antibody) at a dilution of 1/55(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 218282(Anti-WDFY1 Antibody) at dilution 1/55.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 218282(Anti-WDFY1 Antibody) at a dilution of 1/55.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D224088(Anti-WDFY1 Antibody) at dilution 1/55.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse heart tissue lysate;
Primary antibody: 218282(WDFY1 Antibody) at dilution 1/900;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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