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CD86 Rabbit pAb

Anti-CD86 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218448

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218448
Product Name:   Anti-CD86 Rabbit Polyclonal Antibody
Synonyms:   B70; B7-2; B7.2; LAB72; CD28LG2
UNIPROT ID:   P42081 (Gene Accession – BC040261 )
Background:    This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Immunogen:    Fusion protein of human CD86
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 218448(CD86 Antibody) at a dilution of 1/30(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 218448(Anti-CD86 Antibody) at dilution 1/30.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human lymph gland tissue and Jurkat cell lysates;
Primary antibody: 218448(CD86 Antibody) at dilution 1/450;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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