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CHIT1 Rabbit pAb

Anti-CHIT1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218488

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218488
Product Name:   Anti-CHIT1 Rabbit Polyclonal Antibody
Synonyms:   CHI3; CHIT; CHITD
UNIPROT ID:   Q13231 (Gene Accession – BC103695 )
Background:    Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.
Immunogen:    Fusion protein of human CHIT1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 218488(CHIT1 Antibody) at a dilution of 1/30(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 218488(Anti-CHIT1 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 218488(Anti-CHIT1 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with fusion protein and then with D224541(Anti-CHIT1 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human spleen tissue and Human lung tissue lysates;
Primary antibody: 218488(CHIT1 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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