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MRPL1 Rabbit pAb

Anti-MRPL1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218635

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218635
Product Name:   Anti-MRPL1 Rabbit Polyclonal Antibody
Synonyms:   L1MT; BM022; MRP-L1
UNIPROT ID:   Q9BYD6 (Gene Accession – BC014356 )
Background:    Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L1 ribosomal protein family.
Immunogen:    Fusion protein of human MRPL1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218635(MRPL1 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218635(Anti-MRPL1 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: K562, HEPG2, 231 and Jurkat cell lysates;
Primary antibody: 218635(MRPL1 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 7 seconds
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