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HPRT1 Rabbit pAb

Anti-HPRT1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218842

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218842
Product Name:   Anti-HPRT1 Rabbit Polyclonal Antibody
Synonyms:   HPRT; HGPRT
UNIPROT ID:   P00492 (Gene Accession – BC000578 )
Background:    The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
Immunogen:    Fusion protein of human HPRT1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218842(HPRT1 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218842(Anti-HPRT1 Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 293T cell, Mouse brain tissue lysates;
Primary antibody: 218842(HPRT1 Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 40 seconds
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