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MDFI Rabbit pAb

Anti-MDFI Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218845

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218845
Product Name:   Anti-MDFI Rabbit Polyclonal Antibody
Synonyms:   I-MF; I-mfa
UNIPROT ID:   Q99750 (Gene Accession – BC007836 )
Background:    This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.
Immunogen:    Fusion protein of human MDFI
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 218845(MDFI Antibody) at a dilution of 1/55(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 218845(Anti-MDFI Antibody) at dilution 1/55.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 218845(Anti-MDFI Antibody) at a dilution of 1/55.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D225356(Anti-MDFI Antibody) at dilution 1/55.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Human placenta tissue lysate;
Primary antibody: 218845(MDFI Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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