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COPE Rabbit pAb

Anti-COPE Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218863

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218863
Product Name:   Anti-COPE Rabbit Polyclonal Antibody
Synonyms:   epsilon-COP
UNIPROT ID:   O14579 (Gene Accession – BC003155 )
Background:    The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta’, gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
Immunogen:    Fusion protein of human COPE
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 40-200;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218863(COPE Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218863(Anti-COPE Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: HepG2, A375 and PC-3 cell lysates;
Primary antibody: 218863(COPE Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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