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GGA2 Rabbit pAb

Anti-GGA2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218899

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218899
Product Name:   Anti-GGA2 Rabbit Polyclonal Antibody
Synonyms:   VEAR
UNIPROT ID:   Q9UJY4 (Gene Accession – BC000284 )
Background:    This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly.
Immunogen:    Fusion protein of human GGA2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 150-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 218899(GGA2 Antibody) at a dilution of 1/120(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 218899(Anti-GGA2 Antibody) at dilution 1/120.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: HepG2 cell lysate;
Primary antibody: 218899(GGA2 Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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