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H3-3B Rabbit pAb

Anti-H3-3B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218959

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218959
Product Name:   Anti-H3-3B Rabbit Polyclonal Antibody
Synonyms:   H3-3A; H3.3B; H3F3B; BRYLIB2
UNIPROT ID:   P84243 (Gene Accession – BC001124 )
Background:    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17.
Immunogen:    Fusion protein of human H3-3B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 218959(H3-3B Antibody) at a dilution of 1/80(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 218959(Anti-H3-3B Antibody) at dilution 1/80.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: A549, LO2, Raji, 293T cell lysates;
Primary antibody: 218959(H3-3B Antibody) at dilution 1/1200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 15 seconds
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