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GNAI1 Rabbit pAb

Anti-GNAI1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219350

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219350
Product Name:   Anti-GNAI1 Rabbit Polyclonal Antibody
Synonyms:   Gi
UNIPROT ID:   P63096 (Gene Accession – BC026326 )
Background:    Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human GNAI1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 219350(GNAI1 Antibody) at a dilution of 1/130(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 219350(Anti-GNAI1 Antibody) at dilution 1/130.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A172 and Hela cell lysates;
Primary antibody: 219350(GNAI1 Antibody) at dilution 1/2000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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