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POLB Rabbit pAb

Anti-POLB Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219468

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219468
Product Name:   Anti-POLB Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   P06746 (Gene Accession – BC106909 )
Background:    The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]
Immunogen:    Fusion protein of human POLB
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 219468(POLB Antibody) at a dilution of 1/135(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the fusion protein and then with 219468(Anti-POLB Antibody) at dilution 1/135.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 219468(Anti-POLB Antibody) at a dilution of 1/135.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D227211(Anti-POLB Antibody) at dilution 1/135.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: K562, Hela cell lysates;
Primary antibody: 219468(POLB Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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