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CYP51A1 Rabbit pAb

Anti-CYP51A1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219491

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219491
Product Name:   Anti-CYP51A1 Rabbit Polyclonal Antibody
Synonyms:   LDM; CP51; CYP51; CYPL1; P450L1; P450-14DM
UNIPROT ID:   Q16850 (Gene Accession – BC032322 )
Background:    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human CYP51A1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cardiovascular, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 219491(CYP51A1 Antibody) at a dilution of 1/75(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 219491(Anti-CYP51A1 Antibody) at dilution 1/75.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse liver tissue, Rat liver tissue, Hela cell lysates;
Primary antibody: 219491(CYP51A1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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