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SPTLC2 Rabbit pAb

Anti-SPTLC2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219505

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219505
Product Name:   Anti-SPTLC2 Rabbit Polyclonal Antibody
Synonyms:   LCB2; SPT2; HSN1C; LCB2A; NSAN1C; hLCB2a
UNIPROT ID:   O15270 (Gene Accession – BC005123 )
Background:    This gene encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I.
Immunogen:    Fusion protein of human SPTLC2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cardiovascular, Metabolism, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219505(SPTLC2 Antibody) at a dilution of 1/120(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 219505(Anti-SPTLC2 Antibody) at dilution 1/120.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 219505(Anti-SPTLC2 Antibody) at a dilution of 1/120.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D227400(Anti-SPTLC2 Antibody) at dilution 1/120.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A549 and HT29 cell lysates;
Primary antibody: 219505(SPTLC2 Antibody) at dilution 1/900;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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