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RPS10 Rabbit pAb

Anti-RPS10 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219526

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219526
Product Name:   Anti-RPS10 Rabbit Polyclonal Antibody
Synonyms:   S10; DBA9
UNIPROT ID:   P46783 (Gene Accession – BC001032 )
Background:    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternate splicing results in multiple transcript variants that encode the same protein. Naturally occurring read-through transcription occurs between this locus and the neighboring locus NUDT3 (nudix (nucleoside diphosphate linked moiety X)-type motif 3).
Immunogen:    Fusion protein of human RPS10
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 219526(RPS10 Antibody) at a dilution of 1/70(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 219526(Anti-RPS10 Antibody) at dilution 1/70.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: K562 and Raji cell lysates;
Primary antibody: 219526(RPS10 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 40 seconds
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