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DNAJB8 Rabbit pAb

Anti-DNAJB8 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219551

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219551
Product Name:   Anti-DNAJB8 Rabbit Polyclonal Antibody
Synonyms:   DJ6; CT156
UNIPROT ID:   Q8NHS0 (Gene Accession – BC029521 )
Background:    The protein encoded by this gene belongs to the DNAJ/HSP40 family of proteins that regulate chaperone activity. This family member suppresses aggregation and toxicity of polyglutamine proteins, and the C-terminal tail is essential for this activity. It has been implicated as a cancer-testis antigen and as a cancer stem-like cell antigen involved in renal cell carcinoma.
Immunogen:    Fusion protein of human DNAJB8
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 219551(DNAJB8 Antibody) at a dilution of 1/70(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 219551(Anti-DNAJB8 Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 219551(Anti-DNAJB8 Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D227685(Anti-DNAJB8 Antibody) at dilution 1/70.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: A549 cell, Mouse brain tissue, 293T cell lysates;
Primary antibody: 219551(DNAJB8 Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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