Cat.#: S219623 |
Product Name: Anti-RAB17 Rabbit Polyclonal Antibody |
Synonyms: |
UNIPROT ID: Q9H0T7 (Gene Accession – NP_071894 ) |
Background: The Rab subfamily of small GTPases plays an important role in the regulation of membrane trafficking. RAB17 is an epithelial cell-specific GTPase. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. |
Immunogen: Synthetic peptide of human RAB17 |
Applications: ELISA, WB, IHC |
Recommended Dilutions: IHC: 30-150;WB: 200-1000;ELISA: 1000-2000 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Isotype: Immunogen-specific rabbit IgG |
Purification: Antigen affinity purification |
Species Reactivity: Human, Mouse |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
Research Areas: Signal Transduction |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 219623(RAB17 Antibody) at a dilution of 1/40(Cell membrane). | In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 219623(Anti-RAB17 Antibody) at dilution 1/40. | Gel: 12%SDS-PAGE, Lysate: 40 μg; Lane: TM4 cells; Primary antibody: 219623(RAB17 Antibody) at dilution 1/200; Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution; Exposure time: 20 seconds | |
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