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BRAF Rabbit pAb

Anti-BRAF Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219678

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219678
Product Name:   Anti-BRAF Rabbit Polyclonal Antibody
Synonyms:   NS7; BRAF1; RAFB1; B-RAF1
UNIPROT ID:   P15056 (Gene Accession – NP_004324 )
Background:    This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome ,x has been identified for this gene.
Immunogen:    Synthetic peptide of human BRAF
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 219678(BRAF Antibody) at a dilution of 1/30(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 219678(Anti-BRAF Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 219678(Anti-BRAF Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with synthetic peptide and then with D260132(Anti-BRAF Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, Raji cells;
Primary antibody: 219678(BRAF Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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