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CASP12 Rabbit pAb

Anti-CASP12 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219680

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219680
Product Name:   Anti-CASP12 Rabbit Polyclonal Antibody
Synonyms:   CASP-12, CASP12P1
UNIPROT ID:   Q6UXS9 (Gene Accession – NP_001177945 )
Background:    Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene.
Immunogen:    Synthetic peptide of human CASP12
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cell Biology, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219680(CASP12 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 219680(Anti-CASP12 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 219680(Anti-CASP12 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with synthetic peptide and then with D260136(Anti-CASP12 Antibody) at dilution 1/50.

Gel: 10%SDS-PAGE, Lysate: 50 μg;
Lane: NIH/3T3 cells;
Primary antibody: 219680(CASP12 Antibody) at dilution 1/700;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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