Email: info@neweastbio.com   |  Call: 6109452007

CHRM4 Rabbit pAb

Anti-CHRM4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219696

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219696
Product Name:   Anti-CHRM4 Rabbit Polyclonal Antibody
Synonyms:   HM4; M4R
UNIPROT ID:   P08173 (Gene Accession – BC095546 )
Background:    The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition.
Immunogen:    Synthetic peptide of human CHRM4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 219696(CHRM4 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 219696(Anti-CHRM4 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 219696(Anti-CHRM4 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with synthetic peptide and then with D260172(Anti-CHRM4 Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: A549 cell lysate;
Primary antibody: 219696(CHRM4 Antibody) at dilution 1/1600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
          Publications