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HMGB1 Rabbit pAb

Anti-HMGB1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219852

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219852
Product Name:   Anti-HMGB1 Rabbit Polyclonal Antibody
Synonyms:   HMG1; HMG3; HMG-1; SBP-1
UNIPROT ID:   P09429 (Gene Accession – NP_002119 )
Background:    This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
Immunogen:    Synthetic peptide of human HMGB1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using 219852(HMGB1 Antibody) at a dilution of 1/50(Nucleus).

Immunohistochemistry analysis of paraffin-embedded Human cervical cancer tissue using 219852(Anti-HMGB1 Antibody) at a dilution of 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Human 2-3 grade invasive ductal breast tissue, LO2, 293T, Jurkat cell lysates;
Primary antibody: 219852(HMGB1 Antibody) at dilution 1/1200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 seconds
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