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AKAP8 Rabbit pAb

Anti-AKAP8 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219945

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219945
Product Name:   Anti-AKAP8 Rabbit Polyclonal Antibody
Synonyms:   AKAP-8, AKAP95, AKAP 95, AKAP-95
UNIPROT ID:   O43823 (Gene Accession – NP_005849 )
Background:    This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9.
Immunogen:    Synthetic peptide of human AKAP8
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 219945(AKAP8 Antibody) at a dilution of 1/15(cytoplasm, Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 219945(Anti-AKAP8 Antibody) at dilution 1/15.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 219945(Anti-AKAP8 Antibody) at a dilution of 1/15.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D260664(Anti-AKAP8 Antibody) at dilution 1/15.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, 293T cells;
Primary antibody: 219945(AKAP8 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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