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RNF126 Rabbit pAb

Anti-RNF126 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219982

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219982
Product Name:   Anti-RNF126 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q9BV68 (Gene Accession – NP_919442 )
Background:    The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. RNF126 interacts with p21 and RNF126 over-expression increased p21 protein ubiquitination in an E3 ligase activity-dependent manner. RNF126 knockdown induced loss of cell viability in MDA-MB-231 and PC-3 can be partially rescued by depletion of p21. RNF126 stable knockdown in PC3 inhibited tumor growth in SCID mice.
Immunogen:    Synthetic peptide of human RNF126
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 219982(RNF126 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 219982(Anti-RNF126 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 219982(Anti-RNF126 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D260729(Anti-RNF126 Antibody) at dilution 1/20.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Jurkat cells, K562 cells;
Primary antibody: 219982(RNF126 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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