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PRKD3 Rabbit pAb

Anti-PRKD3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220075

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220075
Product Name:   Anti-PRKD3 Rabbit Polyclonal Antibody
Synonyms:   EPK2; PKD3; PRKCN; PKC-NU; nPKC-NU
UNIPROT ID:   O94806 (Gene Accession – NP_005804 )
Background:    Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members.
Immunogen:    Synthetic peptide of human PRKD3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 220075(PRKD3 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 220075(Anti-PRKD3 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 220075(Anti-PRKD3 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D260912(Anti-PRKD3 Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: Human testis tissue, K562 cells, A549 cells, Raji cells, NIH/3T3 cells, Hela cells;
Primary antibody: 220075(PRKD3 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 minutes
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