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MAP2K1 Rabbit pAb

Anti-MAP2K1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220160

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220160
Product Name:   Anti-MAP2K1 Rabbit Polyclonal Antibody
Synonyms:   MEK1, MKK1, MAPKK1, PRKMK1
UNIPROT ID:   Q02750 (Gene Accession – NP_002746.1 )
Background:    The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Immunogen:    Synthetic peptide of human MAP2K1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 220160(MAP2K1 Antibody) at a dilution of 1/25(Cytoplasm, Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 220160(Anti-MAP2K1 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 220160(Anti-MAP2K1 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D261061(Anti-MAP2K1 Antibody) at dilution 1/25.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Jurkat cells, hela cells, K562 cells;
Primary antibody: 220160(MAP2K1 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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