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PAFAH2 Rabbit pAb

Anti-PAFAH2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220163

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220163
Product Name:   Anti-PAFAH2 Rabbit Polyclonal Antibody
Synonyms:   HSD-PLA2
UNIPROT ID:   Q99487 (Gene Accession – NP_000428 )
Background:    This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme.
Immunogen:    Synthetic peptide of human PAFAH2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220163(PAFAH2 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220163(Anti-PAFAH2 Antibody) at dilution 1/80.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: 231 cells;
Primary antibody: 220163(PAFAH2 Antibody) at dilution 1/700;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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