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ATG4A Rabbit pAb

Anti-ATG4A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220314

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220314
Product Name:   Anti-ATG4A Rabbit Polyclonal Antibody
Synonyms:   APG4A; AUTL2
UNIPROT ID:   Q8WYN0 (Gene Accession – NP_443168 )
Background:    Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified.
Immunogen:    Synthetic peptide of human ATG4A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Cell Biology, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220314(ATG4A Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220314(Anti-ATG4A Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220314(Anti-ATG4A Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D261325(Anti-ATG4A Antibody) at dilution 1/30.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 231 cells, human liver cancer tissue;
Primary antibody: 220314(ATG4A Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 minutes
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