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AURKC Rabbit pAb

Anti-AURKC Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220323

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220323
Product Name:   Anti-AURKC Rabbit Polyclonal Antibody
Synonyms:   AIE2; AIK3; ARK3; AurC; SPGF5; STK13; aurora-C
UNIPROT ID:   Q9UQB9 (Gene Accession – NP_001015878 )
Background:    This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants.
Immunogen:    Synthetic peptide of human AURKC
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 15-50;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 220323(AURKC Antibody) at a dilution of 1/15(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 220323(Anti-AURKC Antibody) at dilution 1/15.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Mouse testis tissue, hela cells, human liver cancer tissue, A375 cells, 293T cells;
Primary antibody: 220323(AURKC Antibody) at dilution 1/150;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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