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PIP5K1C Rabbit pAb

Anti-PIP5K1C Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220333

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220333
Product Name:   Anti-PIP5K1C Rabbit Polyclonal Antibody
Synonyms:   LCCS3; PIPKIg_v4; PIP5Kgamma; PIP5K-GAMMA
UNIPROT ID:   O60331 (Gene Accession – NP_036530 )
Background:    This locus encodes a type I phosphatidylinositol 4-phosphate 5-kinase. The encoded protein catalyzes phosphorylation of phosphatidylinositol 4-phosphate, producing phosphatidylinositol 4,5-bisphosphate. This enzyme is found at synapses and has been found to play roles in endocytosis and cell migration. Mutations at this locus have been associated with lethal congenital contractural syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.
Immunogen:    Synthetic peptide of human PIP5K1C
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220333(PIP5K1C Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220333(Anti-PIP5K1C Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220333(Anti-PIP5K1C Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D261375(Anti-PIP5K1C Antibody) at dilution 1/25.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A431 cells, hela cells;
Primary antibody: 220333(PIP5K1C Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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