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COX5A Rabbit pAb

Anti-COX5A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220376

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220376
Product Name:   Anti-COX5A Rabbit Polyclonal Antibody
Synonyms:   VA; COX; COX-VA
UNIPROT ID:   P20674 (Gene Accession – NP_004246 )
Background:    Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities.
Immunogen:    Synthetic peptide of human COX5A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 220376(COX5A Antibody) at a dilution of 1/40(cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 220376(Anti-COX5A Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220376(Anti-COX5A Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D261450(Anti-COX5A Antibody) at dilution 1/40.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cell;
Primary antibody: 220376(COX5A Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 7 seconds
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