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SMAD9 Rabbit pAb

Anti-SMAD9 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220378

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220378
Product Name:   Anti-SMAD9 Rabbit Polyclonal Antibody
Synonyms:   PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B
UNIPROT ID:   O15198 (Gene Accession – NP_001120689 )
Background:    The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human SMAD9
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Cardiovascular, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 220378(SMAD9 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 220378(Anti-SMAD9 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 220378(Anti-SMAD9 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D261453(Anti-SMAD9 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse heart tissue;
Primary antibody: 220378(SMAD9 Antibody) at dilution 1/290;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes
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