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ATR Rabbit pAb

Anti-ATR Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220392

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220392
Product Name:   Anti-ATR Rabbit Polyclonal Antibody
Synonyms:   FRP1; MEC1; SCKL; FCTCS; SCKL1
UNIPROT ID:   Q13535 (Gene Accession – NP_001175 )
Background:    The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.
Immunogen:    Synthetic peptide of human ATR
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 220392(ATR Antibody) at a dilution of 1/70(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 220392(Anti-ATR Antibody) at dilution 1/70.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: LOVO, A549 cell lysates;
Primary antibody: 220392(ATR Antibody) at dilution 1/1350;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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