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ICAM1 Rabbit pAb

Anti-ICAM1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220443

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220443
Product Name:   Anti-ICAM1 Rabbit Polyclonal Antibody
Synonyms:   BB2; CD54; P3.58
UNIPROT ID:   P05362 (Gene Accession – NP_000192 )
Background:    This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Immunogen:    Synthetic peptide of human ICAM1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cardiovascular, Immunology, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 220443(ICAM1 Antibody) at a dilution of 1/40(Cytoplasm and membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 220443(Anti-ICAM1 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 220443(Anti-ICAM1 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with synthetic peptide and then with D261536(Anti-ICAM1 Antibody) at dilution 1/40.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Huvec cells, hela cells, raji cells;
Primary antibody: 220443(ICAM1 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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