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CRKL Rabbit pAb

Anti-CRKL Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220477

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220477
Product Name:   Anti-CRKL Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   P46109 (Gene Accession – NP_005198 )
Background:    This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
Immunogen:    Synthetic peptide of human CRKL
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 220477(CRKL Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 220477(Anti-CRKL Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 220477(Anti-CRKL Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D261585(Anti-CRKL Antibody) at dilution 1/25.

Gel: 15%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Lovo cells, Raji cells, A172 cells;
Primary antibody: 220477(CRKL Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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