Cat.#: S220497 |
Product Name: Anti-DDX4 Rabbit Polyclonal Antibody |
Synonyms: VASA |
UNIPROT ID: Q9NQI0 (Gene Accession – NP_077726 ) |
Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. |
Immunogen: Synthetic peptide of human DDX4 |
Applications: ELISA, IHC |
Recommended Dilutions: IHC: 25-100; ELISA: 1000-2000 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Isotype: Immunogen-specific rabbit IgG |
Purification: Antigen affinity purification |
Species Reactivity: Human, Mouse, Rat |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
Research Areas: Epigenetics and Nuclear Signaling, Stem Cells, Developmental Biology |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human gasrtic cancer tissue using 220497(DDX4 Antibody) at a dilution of 1/25(Cytoplasm). | In comparision with the IHC on the left, the same paraffin-embedded Human gasrtic cancer tissue is first treated with the synthetic peptide and then with 220497(Anti-DDX4 Antibody) at dilution 1/25. | The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220497(Anti-DDX4 Antibody) at a dilution of 1/25. | In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D261611(Anti-DDX4 Antibody) at dilution 1/25. |
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