Email: info@neweastbio.com   |  Call: 6109452007

HSD17B1 Rabbit pAb

Anti-HSD17B1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220612

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220612
Product Name:   Anti-HSD17B1 Rabbit Polyclonal Antibody
Synonyms:   HSD17; EDHB17; EDH17B2; SDR28C1
UNIPROT ID:   P14061 (Gene Accession – NP_000404 )
Background:    Hydroxysteroid (17-beta) dehydrogenase 1 catalyzes the final step in the formation of estradiol and testosterone from estrone and androstenedione, respectively. Ovarian granulosa cells and breast tissue both express HSD17B1. Other tissues that express HSD17B1 include testis, placenta, uterus, prostate and adipose tissue. HSD17B1 functions as a homodimer and prefers NADP(H) over NAD(H) for oxidation and reduction. The gene encoding human HSD17B1 maps to chromosome 17q12-q21. The importance of HSD17B1 to estradiol production suggests the specific inhibition of HSD17B1 may aid in breast cancer therapy.
Immunogen:    Synthetic peptide of human HSD17B1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Signal Transduction, Cancer, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 220612(HSD17B1 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 220612(Anti-HSD17B1 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 220612(Anti-HSD17B1 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D261765(Anti-HSD17B1 Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human placenta tissue;
Primary antibody: 220612(HSD17B1 Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second
          Publications