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ME1 Rabbit pAb

Anti-ME1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220703

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220703
Product Name:   Anti-ME1 Rabbit Polyclonal Antibody
Synonyms:   malic enzyme 1, NADP(+)-dependent, cytosolic
UNIPROT ID:   P48163 (Gene Accession – NP_002386 )
Background:    This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
Immunogen:    Synthetic peptide of human ME1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 220703(ME1 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the synthetic peptide and then with 220703(Anti-ME1 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 220703(Anti-ME1 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D261900(Anti-ME1 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 293T cells, A431 cells;
Primary antibody: 220703(ME1 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 15 seconds
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