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MIG7 Rabbit pAb

Anti-MIG7 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220712

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220712
Product Name:   Anti-MIG7 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q1AHR6 (Gene Accession – ABF71424 )
Background:    MIG-7 protein inhibited protein phosphatase 2A to sustain Akt/GSK-3? phosphorylation and cancer-cell migration/invasion. Cancer cells overexpressing MIG-7 exhibited increased expression of ZEB-1 and Twist in parallel with epithelial-mesenchymal transition, metastasis and cancer lethality. MIG-7 protein level positively correlated with advanced stages of human lung cancers.
Immunogen:    Synthetic peptide of human MIG7
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 220712(MIG7 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 220712(Anti-MIG7 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220712(Anti-MIG7 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D261910(Anti-MIG7 Antibody) at dilution 1/20.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human placenta tissue, Human breast infiltrative duct tissue;
Primary antibody: 220712(MIG7 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 15 seconds
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