Cat.#: S220716 |
Product Name: Anti-MAP3K11 Rabbit Polyclonal Antibody |
Synonyms: MLK3; PTK1; SPRK; MLK-3; MEKK11 |
UNIPROT ID: Q16584 (Gene Accession – NP_002410 ) |
Background: The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. |
Immunogen: Synthetic peptide of human MAP3K11 |
Applications: ELISA, WB, IHC |
Recommended Dilutions: IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Isotype: Immunogen-specific rabbit IgG |
Purification: Antigen affinity purification |
Species Reactivity: Human, Mouse, Rat |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
Research Areas: Signal Transduction |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220716(MAP3K11 Antibody) at a dilution of 1/50(Cytoplasm). | In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220716(Anti-MAP3K11 Antibody) at dilution 1/50. | Gel: 6%SDS-PAGE, Lysate: 40 μg; Lane 1-5: K562, NIH/3T3, PC3, LO2, MCF7 cell lysates; Primary antibody: 220716(MAP3K11 Antibody) at dilution 1/800; Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution; Exposure time: 20 seconds | |
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