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MMP20 Rabbit pAb

Anti-MMP20 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220717

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220717
Product Name:   Anti-MMP20 Rabbit Polyclonal Antibody
Synonyms:   AI2A2; MMP-20
UNIPROT ID:   O60882 (Gene Accession – NP_004762 )
Background:    Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP’s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matri,x and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3.
Immunogen:    Synthetic peptide of human MMP20
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cardiovascular, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 220717(MMP20 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the synthetic peptide and then with 220717(Anti-MMP20 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 220717(Anti-MMP20 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D261915(Anti-MMP20 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse brain tissue, 823 cells;
Primary antibody: 220717(MMP20 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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