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NDUFS2 Rabbit pAb

Anti-NDUFS2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220739

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220739
Product Name:   Anti-NDUFS2 Rabbit Polyclonal Antibody
Synonyms:   CI-49
UNIPROT ID:   O75306 (Gene Accession – NP_004541 )
Background:    The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human NDUFS2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 220739(NDUFS2 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the synthetic peptide and then with 220739(Anti-NDUFS2 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 220739(Anti-NDUFS2 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D261944(Anti-NDUFS2 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse heart tissue, Mouse brain tissue, Jurkat cells;
Primary antibody: 220739(NDUFS2 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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