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PIK3CD Rabbit pAb

Anti-PIK3CD Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220808

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220808
Product Name:   Anti-PIK3CD Rabbit Polyclonal Antibody
Synonyms:   APDS; PI3K; IMD14; p110D; IMD14A; IMD14B; ROCHIS; P110DELTA
UNIPROT ID:   O00329 (Gene Accession – NP_005017 )
Background:    Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I PI3K found primarily in leukocytes. Like other class I PI3Ks (p110-alpha p110-beta, and p110-gamma), the encoded protein binds p85 adapter proteins and GTP-bound RAS. However, unlike the other class I PI3Ks, this protein phosphorylates itself, not p85 protein.
Immunogen:    Synthetic peptide of human PIK3CD
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220808(PIK3CD Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220808(Anti-PIK3CD Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 220808(Anti-PIK3CD Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with synthetic peptide and then with D262049(Anti-PIK3CD Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cell lysate;
Primary antibody: 220808(PIK3CD Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
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