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RNF5 Rabbit pAb

Anti-RNF5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220854

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220854
Product Name:   Anti-RNF5 Rabbit Polyclonal Antibody
Synonyms:   RMA1; RING5
UNIPROT ID:   Q99942 (Gene Accession – NP_008844 )
Background:    The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.
Immunogen:    Synthetic peptide of human RNF5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cell Biology, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220854(RNF5 Antibody) at a dilution of 1/35(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220854(Anti-RNF5 Antibody) at dilution 1/35.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 220854(Anti-RNF5 Antibody) at a dilution of 1/35.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with synthetic peptide and then with D262128(Anti-RNF5 Antibody) at dilution 1/35.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: K562 cells, 231 cells, Jurkat cells, hela cells, 293T cells;
Primary antibody: 220854(RNF5 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes
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