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SMC6 Rabbit pAb

Anti-SMC6 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220953

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220953
Product Name:   Anti-SMC6 Rabbit Polyclonal Antibody
Synonyms:   SMC-6; hSMC6; SMC6L1
UNIPROT ID:   Q96SB8 (Gene Accession – NP_078900 )
Background:    Structural maintenance of chromosomes protein 6 is a protein that in humans is encoded by the SMC6 gene. Core component of the SMC5-SMC6 comple,x a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks.
Immunogen:    Synthetic peptide of human SMC6
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 220953(SMC6 Antibody) at a dilution of 1/20(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 220953(Anti-SMC6 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 220953(Anti-SMC6 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D262276(Anti-SMC6 Antibody) at dilution 1/20.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Jurkat cells, 231 cells, hela cells, 293T cells;
Primary antibody: 220953(SMC6 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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