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NME2 Rabbit pAb

Anti-NME2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221015

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221015
Product Name:   Anti-NME2 Rabbit Polyclonal Antibody
Synonyms:   PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2
UNIPROT ID:   P22392 (Gene Accession – NP_002503 )
Background:    Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of ‘A’ (encoded by NME1) and ‘B’ (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product.
Immunogen:    Synthetic peptide of human NME2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Signal Transduction, Cancer, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 221015(NME2 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 221015(Anti-NME2 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 221015(Anti-NME2 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with synthetic peptide and then with D262361(Anti-NME2 Antibody) at dilution 1/40.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: Jurkat cells, A549 cells, Mouse brain tissue, 293T cells, LNCap cells, NIH/3T3 cells, Hela cells;
Primary antibody: 221015(NME2 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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