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CSPG4 Rabbit pAb

Anti-CSPG4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221052

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221052
Product Name:   Anti-CSPG4 Rabbit Polyclonal Antibody
Synonyms:   NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG
UNIPROT ID:   Q6UVK1 (Gene Accession – NP_001888 )
Background:    A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Immunogen:    Synthetic peptide of human CSPG4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 221052(CSPG4 Antibody) at a dilution of 1/50(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 221052(Anti-CSPG4 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 221052(Anti-CSPG4 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with synthetic peptide and then with D262413(Anti-CSPG4 Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse kidney tissue, Mouse lung tissue, Mouse brain tissue lysates;
Primary antibody: 221052(CSPG4 Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 minutes
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