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ERP44 Rabbit pAb

Anti-ERP44 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221069

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221069
Product Name:   Anti-ERP44 Rabbit Polyclonal Antibody
Synonyms:   PDIA10; TXNDC4
UNIPROT ID:   Q9BS26 (Gene Accession – NP_055866 )
Background:    ERP44 is a 406 amino acid protein that contains one thioredoxin domain, a motif that participates in various redox reactions throughout the cell. Localized to the lumen of the endoplasmic reticulum (ER), TXNDC4 functions to inhibit the activity of IP3R-I (inositol 1,4,5-triphosphate receptor,type 1) within calcium channels. In addition, TXNDC4 is thought to regulate oxidative protein folding within the ER and may be involved in retaining proteins.
Immunogen:    Synthetic peptide of human ERP44
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Metabolism, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221069(ERP44 Antibody) at a dilution of 1/20(Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221069(Anti-ERP44 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 221069(Anti-ERP44 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with synthetic peptide and then with D262437(Anti-ERP44 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: A431 cells, K562 cells, 231 cells, HepG2 cells, Hela cells, A549 cells, Raji cells;
Primary antibody: 221069(ERP44 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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